The SIHR-LC Molecular Biology Lab is a specialised research and diagnostic facility dedicated to advancing molecular diagnostics, effective treatment, and the eventual elimination of leprosy. The laboratory contributes to public health initiatives by providing diagnosis using molecular diagnostic techniques such as Polymerase Chain Reaction (PCR) and Reverse Transcriptase–PCR (RT-PCR).

Beyond diagnostic confirmation, the lab evaluates the viability of Mycobacterium leprae through RT-PCR, enabling a clearer understanding of active infection and disease transmission. Recognising antimicrobial resistance (AMR) as a global challenge, a key research focus involves identifying mutations in specific target genes of M.leprae, facilitating the detection and surveillance of multi-drug resistant (MDR) strains through DNA sequencing. The laboratory also utilises advanced genomic platforms, including Oxford Nanopore Sequencing (GridION and MinION), to support high-resolution pathogen detection, genomic analysis, and translational research.

The lab maintains collaborations with national and international research institutions, healthcare organizations, and academic partners. Through the adoption of new technologies and evolving methodologies, the lab continues to strengthen its role as a reference centre supporting leprosy elimination efforts.

• PCR, Real-Time PCR (qPCR) & RT-PCR
High-sensitivity molecular detection of infectious pathogens and genetic targets, including molecular viability assessment.
• Drug Resistance & Mutation Analysis
Detection of resistance-associated mutations in Mycobacterium leprae and Mycobacterium tuberculosis to support drug resistance surveillance.
• Oxford Nanopore Sequencing (GridION & MinION)
Whole-genome sequencing (WGS) and targeted sequencing for pathogen characterisation and genomic analysis.
• Metagenomic Next-Generation Sequencing (mNGS)
Comprehensive, unbiased sequencing for pathogen detection, microbiome profiling, and mixed-sample analysis.

DNA EXTRACTION & OUTSOURCING SERVICES
• Human Clinical Samples
DNA extraction from blood, swabs, urine, biopsies, slit-skin smears, and culture isolates for molecular diagnostics and sequencing applications.
• Soil Samples
DNA extraction to support environmental monitoring, microbial diversity, and ecological studies.
• Water Samples
DNA extraction for pathogen detection, microbial profiling, and antimicrobial
resistance (AMR) gene surveillance.